Research Microscopy Solutions
台灣（地區)
返回
Research Microscopy Solutions
建議的搜尋
熱門搜尋
ZEISS Volutome
SEMINAR

顯微鏡新智能大視界 Revealing 3D structure via AI-enabled X-ray microscopy and Volume EM

蔡司顯微鏡解決方案事業部是全球唯一提供光學顯微鏡，電子顯微鏡，3D X-ray顯微鏡三大顯微鏡領域的研發製造商。作為全球領先的顯微鏡製造商，蔡司提供啟發靈感的解決方案，不僅限於顯微鏡，還包含軟體及完整的售後服務，致力於幫助使用者的研究發展。

我們尊重客戶的專業，也相當珍惜每次和客戶學習的機會，蔡司台灣和世界各地的工程師保持緊密的聯繫，會彼此分享不同領域的經驗，來提升自我的知識層次，卡爾蔡司誠摯地邀請您參與本次活動，同時能與顯微鏡應用專家們現場交流，活動全程免費，期待您的參與。

  • 3D X-ray顯微鏡與電子顯微鏡關聯應用
  • 電子顯微鏡三維電子顯微成像
  • 智能影像分析

Head of objectives with the 40X-P

誠摯邀請您來參加研討會

本次研討會將完整分享光學顯微鏡、X光學顯微鏡及電子顯微鏡等三大影像應用系統與分析。不論是從釐米至奈米的高度整合與關聯應用的工作流程，還是與搭配對應的影像分析軟體與 AI 影像分析，新型態的應用將大幅拓展生物觀察與生物材料研究的可能性。我們衷心期望將嶄新的應用資訊傳遞給研究人員，期待您的參與。

日期：2023年11月3日(五)
時間：9:30 - 15:00
地點：國立台灣大學生命科學館 演講廳R332

Revealing 3D structure via AI-enabled X-ray microscopy and Volume EM

November 3rd, 2023 | 9:30 - 15:00

Join ZEISS seminar and learn about Laboratory-based X-ray Microscope, Volume EM and arivis Pro ,a powerful software solution for demanding image analysis. The ZEISS Xradia Versa portfolio provides a versatile, best-resolution in its category and contrast platform to allow you to expand the boundaries of your non-destructive sub-micro scale imaging. Serial block-face imaging is a key technology in Volume EM. Explore its applications in life science research and see how it is used in core facilities. Image the ultrastructure of biological, resin-embedded samples in 3D over large areas. ZEISS arivis Pro empowers you to automate image analysis pipelines. Leverage traditional methods or AI models effortlessly to create pipelines for any image size, dimension, or modality without the need to code.

 

  • 地點: 國立臺灣大學生命科學館 NTU TechComm 科技共同空間 R332演講廳
立即註冊
arivis Vision4D

Introducing arivis Pro software

Your Powerful Scientific Imaging Software

arivis Pro is your modular software for multi-channel 2D, 3D and 4D images of almost unlimited size, highly scalable and independent of local system resources. Many modern microscope systems such as high-speed confocal, light sheet, super-resolution, electron microscopy or X-ray instruments can produce huge amounts of imaging data. Handle your datasets without constraints and get your results in next to no time!

For quantification of 3D image data the arivis Analysis Pipeline offers a robust and flexible click-and-play solution for processing and quantification of any kind of multidimensional microscope image data. Start your image analysis today, even if you are not an image analysis expert or programmer.

Using the flexible Analysis Pipeline novices can start with predefined workflows for common use cases, while experts have the flexibility to combine different operators for denoising, segmentation, filtering, and other analysis tasks in a clearly structured pipeline with an interactive preview. The analysis strategy and iterative approach of arivis Pro allows image processing and segmentation of a small field of view, a 3D/4D subset, or the complete data set.

See how arivis Pro works & how you can use it in your lab

Pair it with your microscope and see the difference

  • 被阻止的第三方內容

    由於您的追蹤選項，影片播放器已被阻止。要改變設定和播放影片，請點擊下方的按鈕並同意使用「功能性」追蹤技術。

Experience arivis Pro

How you can use it in your lab

Analysis results can be reviewed in synchronized split view windows in 2D and 3D view simultaneously, which is particularly helpful for densely packed structures and for tracking experiments. The integrated Machine Learning functionality allows the segmentation of even difficult samples easily and without deep knowledge of AI analysis methods.

  • Immediate visualization, annotation and analysis on workstations and notebooks in 2D, 3D and 4D, regardless of image size
  • Advanced and easy-to-use image analysis tools with interactive preview options
  • Easy and Integrated AI with Machine Learning for segmentation, image processing and object classification for quick and reliable results including Deep Learning solutions
  • Open, Scalable and integrated workflows that connect ZEN, ZEISS arivis Cloud, MATLAB and a multitude of Open Source platforms
  • Distance measurements, compartmentalization and classification
  • Easy creation and export of 3D / 4D high resolution images and movies for publication
  • The scientific imaging platform directly integrates with arivis Pro VR for productive and immersive visualization and analysis in Virtual Reality and VisionHub, the scalable solution for data management, storage and processing

Introducing ZEISS arivis Pro VR

  • 被阻止的第三方內容

    由於您的追蹤選項，影片播放器已被阻止。要改變設定和播放影片，請點擊下方的按鈕並同意使用「功能性」追蹤技術。

被阻止的第三方內容

由於您的追蹤選項，影片播放器已被阻止。要改變設定和播放影片，請點擊下方的按鈕並同意使用「功能性」追蹤技術。

Track and proofread your sample

It has never been easier to view your sample and changes in it over time or to make corrections to automated tracking of complex datasets with our improved tracking and proofreading features.

Improved: Manual De-Novo Tracking
Tracking growth or movement over time is a challenge. With improved manual tracking features, arivis Pro VR is the best environment for manual 4D tracking and image analysis.

被阻止的第三方內容

由於您的追蹤選項，影片播放器已被阻止。要改變設定和播放影片，請點擊下方的按鈕並同意使用「功能性」追蹤技術。
3D rendering of individual mitochondria from a glycolytic (left), oxidative (middle), and cardiac (right) muscle FIB-SEM volumes. Various colors represent individual mitochondria. Data collected with ZEISS Crossbeam FIB-SEM. Credit: National Heart, Lung, and Blood Institute at the National Institutes of Health, USA

Subcellular Connectomics

Analyzing Mitochondrial Networks

In C.K.E. Bleck et al. 2018, Dr. Glancy and team applied a connectomics approach using volume electron microscopy with FIB-SEM and SBF-SEM to quantitatively assess the mitochondrial network in cardiac, oxidative, and glycolytic muscle.

They showed that each muscle type, with its differing contraction demands, had different mitochondrial network configurations.  They also assessed mitochondria-lipid droplet interactions and found evidence that individual mitochondria may be tuned to specialize in energy distribution or calcium cycling.

被阻止的第三方內容

由於您的追蹤選項，影片播放器已被阻止。要改變設定和播放影片，請點擊下方的按鈕並同意使用「功能性」追蹤技術。
3D rendering of a region of the fast-twitch myofibrillar matrix showing branching sarcomeres. Data acquired with ZEISS Crossbeam FIB-SEM. Credit: National Heart, Lung, and Blood Institute at the National Institutes of Health, USA

3D Electron Microscopy of Sarcomeres

A Branching Myofibrillar Matrix

In T.B.  Willingham et al. 2020, Dr. Glancy's group uses volume electron microscopy with FIB-SEM to unveil that striated muscle cells form a continuous myofibrillar matrix with frequent, branching sarcomeres. Their work examines changes in branching during postnatal development and in different muscle types. They suggest a new theory for how force is generated based on a mesh-like myofibrillar network rather than many individual, parallel myofibrils.

被阻止的第三方內容

由於您的追蹤選項，影片播放器已被阻止。要改變設定和播放影片，請點擊下方的按鈕並同意使用「功能性」追蹤技術。
3D rendering and rotation of the myosin filaments within a single mouse cardiac sarcomere. Adjacent mitochondria (red), sarcotubular network (green), and a lipid droplet (cyan) are also shown. Data collected with ZEISS Crossbeam FIB-SEM. Credit: National Heart, Lung, and Blood Institute at the National Institutes of Health, USA

3D Modeling of Sarcomere Interactions

Mitochondrial Networks Influences Sarcomere and Myosin Filament Structure

Mitochondria must supply a constant energy stream to actin and myosin filaments within muscle sarcomeres to sustain muscle contractions over time. In P. Katti et al. 2022, 3D electron microscopy is used to examine variations in sarcomere cross-sectional area and provide evidence that both sarcomere structure and myofilament interactions are influenced by the location and orientation of mitochondria within muscle cells.

被阻止的第三方內容

由於您的追蹤選項，影片播放器已被阻止。要改變設定和播放影片，請點擊下方的按鈕並同意使用「功能性」追蹤技術。
3D rendering and fly through of the Drosophila leg muscle myofibrillar network. Data collected with ZEISS Crossbeam FIB-SEM. Credit: National Heart, Lung, and Blood Institute at the National Institutes of Health, USA

3D EM of Drosophila Muscle Types

Analyzing Myofibrillar Connectivity

Continuing their work from T.B. Willingham et al. 2020 mentioned above, Dr. Glancy and team looked into the extent to which myofibrillar connectivity is evolutionarily conserved as well as mechanisms which regulate the specific architecture of sarcomere branching. In P.T. Ajayi et al. 2022,  they present 3D electron microscopy evidence which indicates fruit flies have a myofibrillar connectivity on/off switch that is regulated by both cell-type dependent and independent mechanisms.

被阻止的第三方內容

由於您的追蹤選項，影片播放器已被阻止。要改變設定和播放影片，請點擊下方的按鈕並同意使用「功能性」追蹤技術。

Save Time with Automated Cutting, Image Acquisition, and Pre-processing

Serial block-face imaging requires stable acquisition conditions over a long period of time. ZEISS Volutome allows highly automated and unattended cutting and imaging. The cutting cycle is sped up and image acquisition is accelerated using the dedicated detector ZEISS Volume BSD. During image acquisition, images are simultaneously pre-calculated for stitching and z-stack alignment – meaning results are at your fingertips in one click.

註冊參加 顯微鏡新智能大視界

Breakthrough Imaging with ZEISS Xradia 630 Versa

View highlights of ZEISS Xradia Versa 3D X-ray microscopes: non-destructive imaging, higher resolution with higher throughput.

  • 被阻止的第三方內容

    由於您的追蹤選項，影片播放器已被阻止。要改變設定和播放影片，請點擊下方的按鈕並同意使用「功能性」追蹤技術。
Head of objectives with the 40X-P

Head of objectives with the 40X-P

Breakthrough Resolution Performance to Expand Your Research Horizons

The ZEISS 40X-Prime Objective

With more X-ray photons available on ZEISS Xradia 600-series Versa, you can now achieve even faster time to results for varied samples without compromising resolution. Unique to ZEISS Xradia 630 Versa is the 40X-Prime (40X-P) objective lens.

ZEISS Xradia 630 Versa XRM, with the higher energy capabilities of the exclusive 40X-Prime (40X-P) objective, enables you to push the limits of submicron imaging like never before. Known for their ability to achieve resolution at a Distance (RaaD™), ZEISS Xradia Versa platforms allow high resolution imaging of a wide array of sample types and sizes over a long range of length scales.

With 40X-P, the system achieves unparalleled resolution performance of 450-500 nm across the full range of source voltage, from 30 kV to 160 kV, defining RaaD 2.0. Unlocking entirely new application capabilities for researchers, the ZEISS 40X-P objective enables ZEISS Xradia 630 Versa to push industry standards of submicron imaging resolution.

NavX User Interface

NavX User Interface

NavX User Interface

NavX User Interface

The physics of X-ray imaging can be complex, so ZEISS XRM researchers studied user habits, dove into their challenges, and employed human-centered design (HCD) principles to enable even the newest user in a busy environment to be immediately productive. NavX™, the new user interface for ZEISS Xradia 630 Versa, guides users through automated workflows with intelligent system insights and delivers experimental results more easily and efficiently while also allowing experienced users to explore the full versatility of the platform.

NavX enables you to automate your workflow and provides guidance on the impact the parameters you've chosen will have on your setup. That guidance is directly embedded in the software, taking you through choices in a natural and familiar way.

Additionally, the NavX File Transfer Utility (FTU) takes the data that is being produced by the microscope and automatically transfers it to other locations so that users have their data where they need it, when they need it. These advancements make NavX much more capable for remote operation, advancing user productivity.

NavX intuitive navigation follows the evolution of the XRM user base and revolutionizes X-ray navigation and control with seamless and integrated workflows complementing the planning and execution of advanced correlative workflows..

Flat Panel Extension
Flat Panel Extension

Flat Panel Extension

The flat panel extension (FPX), standard on your ZEISS Xradia 630 Versa X-ray microscope, further increases the versatility of the system, directly supporting the Advanced Reconstruction Toolbox’s AI-based DeepScout for deep learning and neural network training. Leverage FPX to perform a low resolution, large field of view, "scout” scans, and identify interior regions for higher resolution “zoom” scans on a variety of different sample types. The Volume Scout workflow streamlines this process within NavX.

Soy flower
Soy flower

ZEISS DeepScout​ software

Resolution at field of view, throughput at field of view​

ZEISS DeepScout uses high-resolution 3D microscopy datasets as training data for lower resolution, larger field of view datasets and upscales the larger volume data using a neural network model. ZEISS DeepScout, developed through continued algorithmic innovation enabled by the AI infrastructure from ZEISS, employs the unique Scout-and-Zoom capability to acquire richer information at higher resolution, including interior tomographies for large samples. ​

  • Take your large overview scan​
  • Feed it through the ZEISS DeepScout reconstruction algorithm​
  • Get resolution that approaches the resolution of a Zoom scan, but over a much larger field of view. ​

At its core, ZEISS DeepScout relies on the ability to generate multiscale, spatially registered datasets and uses that ability to train neural networks to improve the reconstruction. New capabilities, fueled by deep learning, mitigate the traditional trade-off between field of view and resolution. ​

DeepScout, on the left, shows significantly more cellular information than standard reconstruction, on the right.
Sample courtesy of Keith Duncan, Donal Danforth Plant Science Center.

2023年 11 月 3 日 早上9:30，NTU TechComm 科技共同空間R332演講廳!
Mouse lung​
Mouse lung​ - DeepRecon Pro

ZEISS DeepRecon Pro

Harvest the hidden opportunities in big data generated by your XRM​

The first commercially available deep learning reconstruction technology enables you to increase throughput by up to 10× without sacrificing novel resolution at a distance (RaaD). Alternatively, keep the same number of projections and enhance the image quality further. ZEISS DeepRecon provides significant AI-driven speed or image quality improvement.​

​ZEISS DeepRecon Pro is applicable to both unique samples as well as semi-repetitive and repetitive workflows. Self-train new machine learning network models on-site with an extremely easy-to-use interface. The one-click workflow of ZEISS DeepRecon Pro eliminates the need for a machine learning expert and can be seamlessly operated by even a novice user.​

​ZEISS DeepRecon Pro is now available on ZEISS Xradia Ultra nanoscale XRM.

Mouse lung imaged with Xradia Versa. Sample is iodine stained and captured with 3001 projections.  Reconstruction done using DeepRecon (right). Compared with the equivalent image reconstructed using FDK (left)

Biomedical metal implant in bone. Without MARS, left. With MARS, right.​
Biomedical metal implant in bone. Without MARS, left. With MARS, right.​

Materials Aware Reconstruction Solution (MARS)

Superior image quality for highly attenuating samples​

MARS is a reconstruction algorithm that is aware of the constituents within a reconstruction. A challenge in X-ray reconstruction in a lab setting is that imaging with a polychromatic source creates different X-ray energies to generate a phenomenon called beam hardening. This effect is particularly challenging when your material is very dense and embedded in relatively less dense material. MARS tells the reconstruction system how to compensate for the effect of extreme beam hardening in the regions between very dense objects. This is important in applications like biomaterials, where you might be looking at implants next to bone or tissue. Or electronics where extremely dense solder balls appear next to other less dense materials on a printed circuit board, generating strong artifacts. MARS reconstructs your images to compensate for these effects. ​

Biomedical metal implant in bone. Without MARS, left. With MARS, right.​

Speaker HuaMan Hsu Senior Application Engineer - ZEISS Microscopy

HuaMan is responsible for high-end 3D series systems. She has years of imaging application experiences in key universities Core Facility including National Taiwan University Consortium of Molecular Imaging Key Technology and National Cheng Kung University - Bioimaging Core Facility.

Speaker Nicky Liu Product and Application Sales Specialist - ZEISS Microscopy

Nicky Liu is responsible for applications on ZEISS X-ray microscope (XRM) product portfolio and familiar with Scanning Electron Microscope (SEM). With experienced consultative skills, she can investigate the true problems that customer may have and suggest best usage scenario and product adaptation. Her past experiences working in National Cheng Kung University and Bruker AXS enables her capability to address academic researcher’s and semiconductor industry customers' pain points. Nicky is also the focal point to provide feedback to ZEISS Product Development Team.

SPEAKER Jerry Fan Product and Application Sales Specialist - ZEISS Microscopy

Jerry Fan is responsible for the applications on ZEISS Bio electron microscopy and light microscopy. Prior to Zeiss, Jerry worked for National Taiwan Museum, Imaging Core Facility of National Taiwan University and Nebulum Technologies. His experiences in both LM and SEM enables him to propose versatile workflows for customers . With Zeiss he currently supports the business development of ZEISS Bio-EM and applications of light microscopes.

感謝踴躍註冊

目前名額已滿